Histochemical Study of the Progenetic Trematode Alloglossidium renale
Histochemical staining of healthy A. renale within the host. (a) Alcian blue pH 2.5 staining for acidic mucosubstances. The tegument of the worm stains strongly (arrows) as do the apical and basal borders of the cuboidal antennal gland tubule cells (arrowheads). No metacercarial wall is present. (b) Periodic acid-Schiff staining for neutral carbohydrates reveals uniform staining of A. renale and no metacercarial wall. (c) Ninhydrin-Schiff staining for amino acids reveals light staining of A. renale parenchyma and no metacercarial wall. Arrow: oral sucker; arrowhead: intestinal contents; and double arrowhead: vitellarium. (d) Ferro-ferricyanide staining reveals tyrosine-based material within the vitellarium (double arrowhead) and egg shells (arrow) but no melanin reaction from the host tissue. (a)–(c) Bars = 100 μm. (d) Bar = 200 μm.