Journal of Parasitology Research / 2014 / Article / Tab 2

Research Article

Histochemical Study of the Progenetic Trematode Alloglossidium renale

Table 2

Alloglossidium renale adult worm in situ staining results.

StainTegumentParenchymaVitellariumIntestinal liningOva

Alcian blue (pH 1.0)+++ outer tegument +

Alcian blue (pH 2.5)+++ outer tegument ++

Ferro-ferricyanide++++

Feulgen nuclear reaction++ inner tegument + ++++

Harris hematoxylin+ outer tegument
light brown cytoplasm
+ inner tegument
dark purple nuclei
++ light brown cytoplasm
+ dark purple nuclei
+++ dark purple nuclei+ cytoplasm
++ dark purple nuclei
+ cytoplasm
++ dark purple nuclei

Ninhydrin-Schiff+++ light++++++++

Osmium++ random oil droplets+++

Periodic acid-Schiff+ outer tegument
++ inner tegument
++/−++++

Toluidine blue+ outer tegument light blue
++ inner tegument dark blue (Nuclei)
+ purple nuclei
+ pink acidic structures
+++ dark blue nuclei+ dark blue nuclei
++ light blue acidic structures
+++ dark blue nuclei

Ward’s protein stain+++++/−++++