PCR-Based Detection of Babesia ovis in Rhipicephalus bursa and Small Ruminants

Figure 2

PCR and RFLP profile of amplified 549 bp fragment of the B. ovis-specific ssu rRNA gene. Lane M: 50 bp DNA ladder (Fermentas, Germany), lane 1: undigested PCR product, lane 2: digested PCR product from infected sheep, lane 3: digested PCR products from infected goats, lane 4: tick collected from sheep, lane 5: tick collected from goat, lane 6: tick collected from dogs, lane 7: tick sample after oviposition, lane 8: egg sample, and lane 9: larvae sample.