Partial Purification of Integral Membrane Antigenic Proteins from Trypanosoma evansi That Display Immunological Cross-Reactivity with Trypanosoma vivax
Analysis of T. evansi antigenic polypeptide bands that display cross-reactivity with T. vivax. Purified T. evansi parasites were homogenized to generate the whole-cell extract (H), which was centrifuged to separate the soluble fraction (S) from the particulate fraction (P). Then, P was extracted with 2% Triton X-100, and the Triton X-100 solubilized fraction () was separated from the remaining pellet () by centrifugation. (a) Coomassie blue staining. (b) Immunoblot developed with serum B-303 (Table 1). M = protein molecular weight markers.