Partial Purification of Integral Membrane Antigenic Proteins from Trypanosoma evansi That Display Immunological Cross-Reactivity with Trypanosoma vivax
Reactivity of the T. evansi polypeptide bands contained in Pools I and II against sera from bovines experimentally and naturally infected with T. vivax. Aliquots (400 μL) of Pools I and II (fractions 13–19 and 25–33 from Figure 6, resp.) were separated by electrophoresis on a preparative 12% polyacrylamide slab gel. Following SDS-PAGE, the proteins were electrotransferred to nitrocellulose filters, and the blots were cut into 3 mm strips. Strips containing Pool I (a) or Pool II (b) were developed using positive sera (+) from horses experimentally infected with T. evansi or from bovines experimentally or naturally infected with T. vivax. Parasitologically and serologically negative sera (−) were also employed as controls. In (a), lanes 1–11, sera H-TEVA1, H-TeApEF, B-303, B-103, B-173, B-LC12, B-LC29, B-LC69, B-F5315, B-F5683, and H-LR, respectively. In (b), lanes 1–10, sera H-TEVA1, H-TeApEF, B-303, B-103, B-173, B-LC12, B-LC54, B-F5315, B-F5683, and H-LR, respectively. Sera are described in Tables 1 and 2.