Research Article

In Vitro Detection of Acaricidal Resistance Status of Rhipicephalus (Boophilus) microplus against Commercial Preparation of Deltamethrin, Flumethrin, and Fipronil from North Gujarat, India

Table 1

Dose dependent response of Adult Immersion Test (AIT) and Larval Packet Test (LPT) to various commercial preparations against R. (B.) microplus.

Concentration (ppm)ATR ± SEMA15 ± SEMMR ± SERI ± SEIO (%)Hatching (%) (visual)MDL ± SE (%)

Deltamethrin
Control0.56 ± 0.0206.67 ± 06.670.049 ± 0.0040.0970 ± 0.01000.0010000.8 ± 0.37
300.64 ± 0.0226.67 ± 06.670.048 ± 0.0040.0720 ± 0.00725.778016.2 ± 1.07
350.52 ± 0.0133.33 ± 06.670.031 ± 0.0030.0715 ± 0.00326.288023.0 ± 0.83
500.53 ± 0.0240.00 ± 14.530.037 ± 0.0030.0713 ± 0.00526.498033.8 ± 0.66
700.53 ± 0.0153.33 ± 06.670.037 ± 0.0020.0685 ± 0.00629.388047.8 ± 0.92
1050.58 ± 0.0193.33 ± 06.670.030 ± 0.0030.0507 ± 0.00547.738063.6 ± 0.51

Flumethrin
500.51 ± 0.0166.67 ± 06.670.000 ± 0.0000.000 ± 0.00000.00056.4 ± 0.93
1000.62 ± 0.0373.33 ± 13.330.000 ± 0.0000.000 ± 0.00000.00075.6 ± 1.29
2000.58 ± 0.0293.33 ± 06.670.000 ± 0.0000.000 ± 0.00000.00092.82 ± 1.24
4000.63 ± 0.0293.33 ± 06.670.000 ± 0.0000.000 ± 0.00000.000100.0 ± 0.00
6000.51 ± 0.01100.00 ± 00.000.000 ± 0.0000.000 ± 0.00000.000100.0 ± 0.00

Fipronil
Control0.51 ± 0.0100.00 ± 00.000.050 ± 0.0010.098 ± 0.00200.0010001.2 ± 0.37
80.57 ± 0.0273.33 ± 06.670.000 ± 0.0000.000 ± 0.00000.00063.4 ± 0.68
100.58 ± 0.0286.66 ± 06.670.000 ± 0.0000.000 ± 0.00000.00085.8 ± 1.28
120.56 ± 0.0293.33 ± 06.670.000 ± 0.0000.000 ± 0.00000.000100.0 ± 0.00
160.50 ± 0.0193.33 ± 06.670.000 ± 0.0000.000 ± 0.00000.000100.0 ± 0.00
180.54 ± 0.02100.00 ± 00.000.000 ± 0.0000.000 ± 0.00000.000100.0 ± 0.00
240.57 ± 0.01100.00 ± 00.000.000 ± 0.0000.000 ± 0.00000.000100.0 ± 0.00

ATR: average tick weight per replicate; SE: standard error; MA15: mean% adult mortality within 15 days; MMR: mean eggs mass per replicate; RI: reproductive index; IO (%): percent inhibition of oviposition; MDL (%): mean dead larva
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