Could kDNA-PCR in Peripheral Blood Replace the Examination of Bone Marrow for the Diagnosis of Visceral Leishmaniasis?
Table 3
Comparative analysis between the results of PB kDNA-PCR and results of BM kDNA-PCR, BM-S, BM-C, and PB-rk39.
Test
PB kDNA-PCR
Kappa (CI 95%)
value
Efficiency (%) (CI 95%)
Positive
Negative
Total
BM kDNA-PCR
0.947
<0.001
97.4 (86.2–99.9)
Positive
19
0
19
(0.846–1.000)
Negative
1
18
19
Almost perfect correlation
Total
20
18
38
BM-S
0.791
<0.001
89.5 (75.2–97.1)
Positive
16
0
16
(0.602–0.980)
Negative
4
18
22
Good concordance
Total
20
18
38
BM-C
0.387
0.001
68.4 (51.3–82.5)
Positive
8
0
8
(0.160–0.614)
Negative
12
18
30
Low concordance
Total
20
18
38
PB-rK39
0.740
<0.001
86.4 (71.9–95.6)
Positive
15
0
15
(0.534–0.945)
Negative
5
18
23
Good concordance
Total
20
18
38
BM kDNA-PCR: kDNA PCR in sample from bone marrow; PB kDNA-PCR: kDNA PCR in sample from peripheral blood; PB-rk39: rK39 with sample from peripheral blood; CI 95%: 95% confidence intervals.
