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Journal of Parasitology Research
Volume 2016 (2016), Article ID 3074803, 7 pages
http://dx.doi.org/10.1155/2016/3074803
Research Article

Genetic Polymorphism of msp1 and msp2 in Plasmodium falciparum Isolates from Côte d’Ivoire versus Gabon

1Malaria Research and Control Centre, National Institute of Public Health, BPV 47, Abidjan, Côte d’Ivoire
2Faculty of Pharmacy, Department of Parasitology and Mycology, Félix Houphouët-Boigny University, BPV 34, Abidjan, Côte d’Ivoire
3Faculty of Medicine, Department of Parasitology and Mycology, University des Sciences de la Santé, BP 4009, Libreville, Gabon
4Parasitology and Mycology Laboratory of Diagnosis and Research Centre on AIDS and Other Infectious Diseases, 01 BPV 03, Abidjan, Côte d’Ivoire

Received 10 December 2015; Revised 1 March 2016; Accepted 7 March 2016

Academic Editor: José F. Silveira

Copyright © 2016 William Yavo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Introduction. The characterization of genetic profile of Plasmodium isolates from different areas could help in better strategies for malaria elimination. This study aimed to compare P. falciparum diversity in two African countries. Methods. Isolates collected from 100 and 73 falciparum malaria infections in sites of Côte d’Ivoire (West Africa) and Gabon (Central Africa), respectively, were analyzed by a nested PCR amplification of msp1 and msp2 genes. Results. The K1 allelic family was widespread in Côte d’Ivoire (64.6%) and in Gabon (56.6%). For msp2, the 3D7 alleles were more prevalent (>70% in both countries) compared to FC27 alleles. In Côte d’Ivoire, the frequencies of multiple infections with msp1 (45.1%) and msp2 (40.3%) were higher than those found for isolates from Gabon, that is, 30.2% with msp1 and 31.4% with msp2. The overall complexity of infection was 1.66 (SD = 0.79) in Côte d’Ivoire and 1.58 (SD = 0.83) in Gabon. It decreased with age in Côte d’Ivoire in contrast to Gabon. Conclusion. Differences observed in some allelic families and in complexity profile may suggest an impact of epidemiological facies as well as immunological response on genetic variability of P. falciparum.