Construction of Two mCherry Plasmids (pXG-mCherry) for Transgenic Leishmania: Valuable Tools for Future Molecular Analysis

Table 1

Oligonucleotides used in this work.

Oligonucleotide	Sequence ()
CH01 (Fw)	g aGC GGC CGC gaT CTA GAc atg gtg agc aag ggc ga
CH02 (Rv)	c tGG ATC Cct cga gct tta ctt gta cag ctc gtc cat
CH03 (Rv)	a tGC GGC CGC tcg agt ctt gta cag ctc gtc cat gc
CH04 (Fw)	g GGA TCC TCT AGA GCc atg gtg agc aag ggc gag ga
LPG1-Fw	at GCG GCC GCc acc atg gcg cct cgt cgc tgg cat
LPG1-Rv	ga GCG GCC GC tta gct agg atc aac agc aaa

Note. Uppercase letters represent added restriction enzyme sites. In bold is the mCherry/LPG1 gene sequence.