Selecting PCR for the Diagnosis of Intestinal Parasitosis: Choice of Targets, Evaluation of In-House Assays, and Comparison with Commercial Kits

Table 1

Primer and probes used for in-house real-time PCR assays in study.

Parasites	Forward primer sequence, 5′→3′	Target	Reference
	Reverse primer sequence, 5′→3′
	Probe sequence (FAM), 5′→3′
Giardia lamblia	GAC GGC TCA GGA CAA CGG TT; TTG CCA GCG GTG TCC G; CCC GCG GCG GTC CCT GCT AG	ssu-rRNA	Verweij et al.; (2004) [1]
C. parvum/C. hominis; (assay 1)	CTT TTT ACC AAT CAC AGA ATC ATC AGA; TGT GTT TGC CAA TGC ATA TGA A; TCG ACT GGT ATC CCT ATA A	DNA J-like protein gene	Bruijnesteijn  van  Coppenraet  et al.; (2009) [4]
C. parvum/C. hominis; (assay 2)	CGC TTC TCT AGC CTT TCA TGA; CTT CAC GTG TGT TTG CCA AT; CCA ATC ACA GAA TCA TCA GAA TCG ACT GGT ATC	DNA J-like protein gene	Fontaine  and  Guillot; (2002) [14]
C. parvum/C. hominis; (assay 3)	GAA CTG TAC AGA TGC TTG GGA GAA T; CCTT CGT TAG TTG AAT CCT CTT TCC A; TTG GAG CTC ATA TCA G; C. hominis probe; CTT GGA GCT CGT ATC AG; C. parvum probe	Specific protein-coding gene	Yang et al.; (2013) [15]
Entamoeba histolytica	ATT GTC GTG GCA TCC TAA CTC A; GCG GAC GGC TCA TTA TAA CA; CAT TGA ATG AAT TGG CCA TT	ssu-rRNA	Verweij et al.; (2004) [1]; Stensvold et al.; (2010) [16]
Internal control
Phocine herpesvirus (PhHV)	GGG CGA ATC ACA GAT TGA ATC; GCG GTT CCA AAC GTA CCA A; TTT TTA TGT GTC CGC CAC CAT CTG GAT C	Glycoprotein B	Niesters; (2001) [13]