Table 1: Primer and probes used for in-house real-time PCR assays in study.

ParasitesForward primer sequence, 5′→3′Target Reference
Reverse primer sequence, 5′→3′
Probe sequence (FAM), 5′→3′

Giardia lambliaGAC GGC TCA GGA CAA CGG TT
TTG CCA GCG GTG TCC G
CCC GCG GCG GTC CCT GCT AG
ssu-rRNAVerweij et al.
(2004) [1]

C. parvum/C. hominis  
(assay 1)
CTT TTT ACC AAT CAC AGA ATC ATC AGA
TGT GTT TGC CAA TGC ATA TGA A
TCG ACT GGT ATC CCT ATA A
DNA J-like protein geneBruijnesteijn  van  Coppenraet  et al.  
(2009) [4]

C. parvum/C. hominis   
(assay 2)
CGC TTC TCT AGC CTT TCA TGA
CTT CAC GTG TGT TTG CCA AT
CCA ATC ACA GAA TCA TCA GAA TCG ACT GGT ATC
DNA J-like protein geneFontaine  and  Guillot  
(2002) [14]

C. parvum/C. hominis   
(assay 3)
GAA CTG TAC AGA TGC TTG GGA GAA T
CCTT CGT TAG TTG AAT CCT CTT TCC A
TTG GAG CTC ATA TCA G  
C. hominis probe
CTT GGA GCT CGT ATC AG  
C. parvum probe
Specific protein-coding geneYang et al.
(2013) [15]

Entamoeba histolyticaATT GTC GTG GCA TCC TAA CTC A
GCG GAC GGC TCA TTA TAA CA
CAT TGA ATG AAT TGG CCA TT
ssu-rRNAVerweij et al.
(2004) [1]
Stensvold et al.  
(2010) [16]

Internal control

Phocine herpesvirus (PhHV)GGG CGA ATC ACA GAT TGA ATC
GCG GTT CCA AAC GTA CCA A
TTT TTA TGT GTC CGC CAC CAT CTG GAT C
Glycoprotein BNiesters  
(2001) [13]