Development and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Diagnosis of Schistosoma mansoni Infection in Faecal Samples

Figure 2

(a) Lane 2 represent S. mansoni adult worm DNA (positive control), lanes 3 and 4 represent results of DNA amplification in faecal samples with high intensity S. mansoni infection (>400 eggs/g faeces), lanes 5 and 6 represent amplification results of faecal samples with moderate infection intensities (100-399 eggs/g faeces), lanes 7 and 8 represent results of faecal samples with low infection intensities (<100 eggs/g faeces), lane 9 represents amplification results from faecal sample from a non-S. mansoni infected subject (negative control), and lanes 1 and 10 represent the molecular marker. (b) LAMP amplification reactions in a tube with products stained with SYBR Green I stain, and samples visualized directly with unaided eye. Tubes 1-8 in Figure 2(b) correspond to lanes 2-9, respectively, in Figure 2(a).

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