“CLUPS”: A New Culture Medium for the Axenic Growth of Entamoeba histolytica

Table 1

Composition of the culture media, PEHPS, TYI-S-33, and CLUPS.

Component	Brand	PEHPS [9]	TYI-S-33	CLUPS
Liver and pancreas extract (mL)	In-house	250	None	None
Lung and pancreas extract (mL)	In-house	None	None	250
Liver infusion broth (g)	BD Difco	None	10	None
Casein peptone (g)	BD Bioxon	10	20	10
Ascorbic acid (g)	Sigma Aldrich	0.2	1	1
Cysteine (g)	Sigma Aldrich	1.0	1	1.26
Glucose (g)	Tecnica quimica	6	10	6
K2HPO4 (g)	Baker	1	1	1
KH2PO4 (g)	Monterrey	0.6	0.6	0.6
Sodium chloride (g)	Baker	None	3.3	None
Ferric citrate (g)	Sigma Aldrich	None	0.02	None
Deionized water		Up to 1L	Up to 1L	Up to 1L