Localisation of TcNaa30 and TcNaa10/TcNaa15 in T. cruzi. (a) TcNaa30 localization in epimastigotes by western blotting. (b) Localization of TcNaa10/TcNaa15. Cytoplasmic (Cyt) and nuclear (N) fractions were assessed for the presence of TcNaa30, TcNaa10, and TcNaa15. Anticyclophilin A and antihistone 3 were used as positive controls for cytoplasmic and nuclear protein, respectively. T/L indicates total cell lysate.