Analysis of the IGS rRNA Region and Applicability for Leishmania (V.) braziliensis Characterization

Figure 3

Reaction efficiency with the Lb/IGS system shown in the standard curve. Standard curve resulting from the amplification reaction of Leishmania braziliensis (Lb) DNA in six points or concentrations, demonstrating a detection limit of up to 10 fg for this specie. On the ordinate axis is the threshold cycle or cut-off point (), and on the abscissa axis is the amount of template DNA in fentograms (fg). IGS—Intergenic Spacer; rRNA—ribosomal RNA.