Analysis of the IGS rRNA Region and Applicability for Leishmania (V.) braziliensis Characterization

Table 2

Characteristics of primers designed for Leishmania spp. IGS rRNA region amplification.

Genome location	Primer	Size	% C/G
L. major region 28S rRNA end	28S F 5-GAGGCCTGAAATTTCATGCTC-3	21 bases	47.62%	62°C
L. major region 18S rRNA start	18S R 5-GATCTGGTTGATTCTGCCAG-3	20 bases	50%	60°C

IGS—Intergenic Spacer; rRNA—ribosomal RNA; % C/G—cytosine/guanine percentage; —melting temperature.