Optimization of a Loop-Mediated Isothermal Amplification Assay as a Point-of-Care Tool for the Detection of Wuchereria bancrofti in Human Blood in Tana River Delta, Kenya

Figure 4

(a) LAMP reaction mix before amplification. (b) LAMP mix reaction after amplification. (a) All reaction mix in tubes 1–5 was blue in color before incubation. On incubating the reaction mix at 63°C for 60 minutes, the results were as shown in (b) on the addition of 1 : 10 SYBR Green dye and viewed under a UV Light or under sunlight. Tubes 1 and 2 remained blue in color indicating the absence of Wuchereria bancrofti DNA (negative specimen), tube 3 turned green in color indicating positive W. bancrofti DNA amplification (positive specimen), tube 4 contained W. bancrofti DNA-positive control which turned green in color, and tube 5 was a negative control which remained blue in color.

(a)

(b)