Optimization of a Loop-Mediated Isothermal Amplification Assay as a Point-of-Care Tool for the Detection of Wuchereria bancrofti in Human Blood in Tana River Delta, Kenya

Figure 5

LAMP sensitivity testing on gel electrophoresis. Lane NC represents negative control; lane PC represents positive control; lanes 10⁻¹–10⁻⁶ represented serial dilutions of DNA extracts for testing the assay sensitivity. 10⁻¹–10⁻⁶ showed amplification of W. bancrofti as bands appeared at different lengths indicating that our LAMP assay was sensitive and could detect to up to 1/1000000 (1/10⁶) DNA copies of the parasites in the diluent, while no band was observed in 1/10000000 (1/10⁷) copies in the diluent.