Research Article

Functional Metagenomics Characterization of an Anaerobic Saltwater Bioreactor

Figure 5

Neighbor joining tree of bioreactor meta-metabolic networks. A neighbor joining tree of ten shotgun metagenomic datasets obtained from anaerobic bioreactors (Supplementary Table 6) was constructed. First, individual sequence reads were Blast searched against the NCBI nonredundant database, and then the identified enzyme homologs were ID mapped to K numbers, which were then used to construct a meta-metabolic network. The network edges were aligned, and distance was calculated between each pair of networks. The resulting pairwise distance matrix was then used to construct a neighbor joining tree. The distance represents D. Further details are in Methods.