Monitoring of Enzymatic Proteolysis Using Self-Assembled Quantum Dot-Protein Substrate Sensors
Figure 3
(a) Results from assaying an increasing concentration of papain
against a constant amount of QD-MyG-Cy3 in the absence
(blue squares) and in presence (red circles) of 10 mM AIA (red
squares) or 1 μM pepstatin (purple triangles) inhibitors. Changes
in FRET efficiency were converted to activity (nM MyG substrate
cleaved/min) as described in the text. (b) Velocity
versus increasing Pro-K concentration assayed against a fixed
QD-MBP-Cy3 substrate concentration in the absence (blue squares)
and presence of 426 μM of kanamycin inhibitor (red circles).
Solid lines are best fits to the data to aid the eye.
