Detection range of the miRNA biosensor assay with dynamic hybridization. (a) Total RNA extracted from MCF-7 cells was serially 10-fold diluted. Different amounts of miR-145 could be detected by the biosensor loaded without or with target miRNAs derived from 10 cells, cells, cells, cells, cells, and cells reaction at , respectively. (b) Correlation between the rate of fluorescence changes and molecular amounts of miRNAs. The mean value was calculated from three independent detection experiments. Error bars show variations among the experiments as the standard deviation of the mean.
