Quartz Crystal Microbalance as a Sensor to Characterize Macromolecular Assembly Dynamics
Figure 16
Structural transformation from intact vesicles to a lipid bilayer on a gold substrate mediated by an amphipathic -helix peptide (AH) peptide. (a) Change in QCM resonant frequency and dissipation as a function of time for intact vesicles on oxidized gold. (B) Structural transformation from intact vesicles to a lipid bilayer (blue curve) and (red curve) show vesicle adsorption on oxidized gold. After 10 minutes (arrow 1) of stabilizing the frequency signal, a POPC vesicle solution (0.1 mg/mL, diameter = 59 nm ± 0.2 nm) was injected into the liquid cell. After 50 and 55 minutes (arrows 2 and 3), the same buffer was used for two washes and the stability of the intact vesicle adlayer on the gold surface was observed. (b) At 60 minutes (arrow 4), an AH peptide solution was added (0.05 mg/mL) to the intact vesicle adlayer on the gold surface. After 120 and 140 minutes (arrows 5 and 6), the same buffer was used for two washes and the stability of the bilayer formed on the gold surface was observed. (c) Illustration of vesicle rupture and fusion processes to form a supported bilayer on a gold substrate. Reprint from [35].
