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Journal of Sensors
Volume 2010 (2010), Article ID 143173, 9 pages
Research Article

Rapid Screening of Alicyclobacillus acidoterrestris Spoilage of Fruit Juices by Electronic Nose: A Confirmation Study

1SSICA-Stazione Sperimentale per l'Industria delle Conserve Alimentari, Viale Tanara, 31/a, 43100 Parma, Italy
2SENSOR Laboratory, Department of Chemistry and Physics for Engineering and Materials, Brescia University and CNR-IDASC, via Valotti 9, I-25123 Brescia, Italy
3Dipartimento di Scienze Agrarie e degli Alimenti, Università degli Studi di Modena e Reggio Emilia, via Giovanni Amendola, 2 Padiglione Besta, 42122 Reggio Emilia, Italy

Received 23 September 2010; Accepted 1 November 2010

Academic Editor: K. Kalantar-Zadeh

Copyright © 2010 Stefano Cagnasso et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Early screening of Alicyclobacillus spp. in fruit juices is a major applicative goal for the food industry, since juice contamination can lead to considerable loss of quality, and subsequently, to economic damages for juice producers. This paper presents an accurate study to assess and confirm the EOS507 electronic nose's (EN) ability of diagnosing Alicyclobacillus acidoterrestris spoilage in artificially contaminated fruit juices. The authors experimental results have shown that the EOS507 can early identify, just after 24 hours from inoculation, contaminated orange and pear juices with an excellent classification rate close to 90% and with a detection threshold as low as 103 cfu/ml. In apple juice the detection threshold was about 105 cfu/ml, thus requiring longer incubation times (72 hours). PLS regression of EOS507 data can be also used to predict with fair accuracy the colony-forming units concentration of the bacteria. These results were supported by the GC/MS/MS measurements of specific chemical markers, such as guaiacol.