Improvement of Accuracy in Flow Immunosensor System by Introduction of Poly-2-[3-(methacryloylamino)propylammonio]ethyl 3-aminopropyl Phosphate

Table 1

Comparison of the slopes and variations obtained from calibration curves of the sensor system.

Method	Blocking			Immobilization
	None	BSA	Poly-MPC	BSA	Poly-3MAm3AP	Poly-3AAm1BA
Anti-LH	0.214	0.158	0.168	0.138	0.246	0.298
Variation*	±0.112	±0.121	±0.087	±0.080	±0.032	±0.054
Anti-hemo	0.133	0.074	0.104	0.078	0.014	0.021
Ratio**	0.622	0.468	0.619	0.565	0.057	0.070

*Average relative deviation obtained from calibration plot of anti-LH.
**Anti-hemo/Anti-LH.