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Journal of Sensors
Volume 2012, Article ID 217958, 6 pages
http://dx.doi.org/10.1155/2012/217958
Research Article

Measurement of Hepatitis B Surface Antigen Concentrations Using a Piezoelectric Microcantilever as a Mass Sensor

1Department of Mechanical Engineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea
2Corporate Technology Operations SAIT, Samsung Electronics Co., Ltd., Yongin 446-712, Republic of Korea
3Department of Chemical Engineering, Pohang University of Science and Technology, Pohang 790-784, Republic of Korea

Received 15 June 2011; Accepted 28 July 2011

Academic Editor: Martin Hegner

Copyright © 2012 Sangkyu Lee et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Hepatitis B surface antigen (HBsAg) concentrations were measured using a piezoelectric microcantilever sensor (PEMS) developed by the authors. The developed PEMS is label-free and detects the sensing signal electrically. It was designed to measure the mass of biomolecules attached to it using an accurate mass-microbalancing technique; its probe area is confined to the end of the cantilever, and its equivalent spring constant is relatively high to minimize the effect of changes in the surface stress when the biomolecules are attached to it. The “dip- and-dry” technique was used to enable the probe area of the sensor to react with reagents in controlled environmental conditions. HBsAg was detected by an immunoreaction whereas the reaction time, antibody density, and its area on the probe were kept at a constant level. The mass of the detected HBsAg was measured in the range of 0.1–100 ng/mL.