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Journal of Sensors
Volume 2016 (2016), Article ID 5653975, 9 pages
http://dx.doi.org/10.1155/2016/5653975
Research Article

Voltammetric Determination of Epinephrine in Pharmaceutical Sample with a Tyrosinase Nanobiosensor

1Division of Chemistry Education, Buca Faculty of Education, Dokuz Eylül University, Buca, 35150 İzmir, Turkey
2Department of Biotechnology, Institute of Natural Science, Dokuz Eylül University, Buca, 35390 İzmir, Turkey

Received 20 April 2016; Revised 11 July 2016; Accepted 13 July 2016

Academic Editor: Junhang Dong

Copyright © 2016 Şenol Alpat et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A novel carbon paste electrode modified with a multiwalled carbon nanotube (MWCNT), tyrosinase, and Nafion membrane (CP/MWCNT/Tyr/Nafion) was developed for voltammetric determination of epinephrine (EP). The CP/MWCNT/Tyr/Nafion biosensor exhibited linear dynamic range from  M to  M EP concentration with a good correlation coefficient (). The detection limit of the biosensor was calculated as  M EP from the signal-to-noise ratio (S/N = 3). Reproducibility of the biosensor was also calculated from relative standard deviation as 3.8% (). Ascorbic acid (AA) and uric acid (UA) did not interfere in the quantification of epinephrine. The developed biosensor was also successfully applied for the determination of epinephrine in pharmaceutical sample. The CP/MWCNT/Tyr/Nafion biosensor has good sensitivity, selectivity, stability, easy preparation procedure, and short analysis time and can be used for the determination of EP in pharmacological samples.