Table of Contents Author Guidelines Submit a Manuscript
Journal of Sensors
Volume 2016, Article ID 6070742, 7 pages
Research Article

DNA Hybridization Detection Based on Resonance Frequency Readout in Graphene on Au SPR Biosensor

1Department of Electrical and Electronic Engineering (EEE), Rajshahi University of Engineering and Technology (RUET), Rajshahi 6204, Bangladesh
2Faculty of Engineering and IT, University of Technology Sydney (UTS), Sydney, NSW 2007, Australia

Received 27 August 2015; Revised 19 October 2015; Accepted 25 October 2015

Academic Editor: Zhi-Mei Qi

Copyright © 2016 Md. Biplob Hossain and Md. Masud Rana. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This paper demonstrates a numerical modeling of surface plasmon resonance (SPR) biosensor for detecting DNA hybridization by recording the resonance frequency characteristics (RFC). The proposed sensor is designed based on graphene material as biomolecular recognition elements (BRE) and the sharp SPR curve of gold (Au). Numerical analysis shows that the variation of RFC for mismatched DNA strands is quiet negligible whereas that for complementary DNA strands is considerably countable. Here, graphene is used to perform faster immobilization between target DNA and probe DNA. The usage of graphene also changes the RFC that ensure hybridization of DNA event by utilizing its optochemical property. In addition, proposed sensor successfully distinguishes between hybridization and single-nucleotide polymorphisms (SNP) by observing the variation level of RFC and maximum transmittance. Therefore, the proposed frequency readout based SPR sensor could potentially open a new window of detection for biomolecular interactions. We also highlight the advantage of using graphene sublayer by performing the sensitivity analysis. Sandwiching of each graphene sublayer enhances 95% sensitivity comparing with conventional SPR sensor.