Determination of Different Saccharides Concentration by Means of a Multienzymes Amperometric Biosensor

Table 1

Working parameters of biosensors for concentration determination of lactose and glucose. The asterisk means that the numerical values of sensitivity were evaluated from published linear calibration. n.a. stands for not available.

Immobilization support & method	Substrate	Linear range (mM)	LOD (mM)	Sensitivity	Stability	References
Triacetyl cellulose membranes	Lactose; Glucose	Up to 180; Up to 5	n.a.; n.a.	~4 10^−3 μA/; n.a.	More than 10 days	[25]
Carbon paste	Lactose; Glucose	Up to 2.5; Up to 2.5	0.1; 0.05	5.6 10^−2 μA/mM; n.a.	More than 10 days	[26]
β-Cyclodextrin polymer	Lactose; Glucose	Up to 4; n.a.	n.a.; n.a.	~4 10^−1 μA/; n.a	Two months	[27]
Conductometric transducer using gold electrodes	Lactose; Glucose	Up to 1–3; Up to 1–3	0.001; 0.001	~12 μS/; n.a.	Four months	[28]
Graphite electrode	Lactose; Glucose	Up to 1.0 10^−2; Up to 1.5 10^−2	0.001; 0.001	850 ± 81 μA/mM; 505 ± 55 μA/mM	Two months	Present work