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Journal of Skin Cancer
Volume 2011 (2011), Article ID 574695, 6 pages
http://dx.doi.org/10.1155/2011/574695
Research Article

Characterization of Ex Vivo Expanded Tumor Infiltrating Lymphocytes from Patients with Malignant Melanoma for Clinical Application

1Center for Cancer Immune Therapy (CCIT), Department of Oncology, University Hospital Herlev, Herlev Ringvej 75, 2730 Herlev, Denmark
2Center for Cancer Immune Therapy (CCIT), Department of Hematology, University Hospital Herlev, Herlev Ringvej 75, 2730 Herlev, Denmark

Received 4 February 2011; Accepted 16 April 2011

Academic Editor: Giuseppe Palmieri

Copyright © 2011 Niels Junker et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Clinical trials of adoptive transfer of autologous tumor infiltrating lymphocytes (TILs) to patients with advanced malignant melanoma have shown remarkable results with objective clinical responses in 50% of the treated patients. In order to initiate a clinical trial in melanoma, we have established a method for expanding TILs to clinical relevant quantities in two steps with in 8 weeks. Further characterization of expanded TILs revealed an oligoclonal composition of T-cells with an effector memory like phenotype. When autologous tumor was available, TILs showed specific activity in all patients tested. TIL cultures contained specificity towards tumor cells as well as peptides derived from tumor-associated antigens (TAAs) during expansion procedures.