Review Article | Open Access
Yutaka Ueda, Takayuki Enomoto, Toshihiro Kimura, Kiyoshi Yoshino, Masami Fujita, Tadashi Kimura, "Two Distinct Pathways to Development of Squamous Cell Carcinoma of the Vulva", Journal of Skin Cancer, vol. 2011, Article ID 951250, 7 pages, 2011. https://doi.org/10.1155/2011/951250
Two Distinct Pathways to Development of Squamous Cell Carcinoma of the Vulva
Squamous cell carcinoma (SCC) accounts for approximately 95% of the malignant tumors of the vaginal vulva and is mostly found in elderly women. The future numbers of patients with vulvar SCC is expected to rise, mainly because of the proportional increase in the average age of the general population. Two different pathways for vulvar SCC have been put forth. The first pathway is triggered by infection with a high-risk-type Human Papillomavirus (HPV). Integration of the HPV DNA into the host genome leads to the development of a typical vulvar intraepithelial neoplasia (VIN), accompanied with overexpression of and . This lesion subsequently forms a warty- or basaloid-type SCC. The HPV vaccine is a promising new tool for prevention of this HPV related SCC of the vulva. The second pathway is HPV-independent. Keratinizing SCC develops within a background of lichen sclerosus (LS) through a differentiated VIN. It has a different set of genetic alterations than those in the first pathway, including p53 mutations, allelic imbalances (AI), and microsatellite instability (MSI). Further clinical and basic research is still required to understand and prevent vulvar SCC. Capsule. Two pathway for pathogenesis of squamous cell carcinoma of the value are reviewed.
Squamous cell carcinoma (SCC) accounts for only 5% of the malignant tumors of the female genital tract, but it represents 95% of vaginal vulvar tumors . The incidence of malignant vulvar tumors in the United States is 1.5 per 100,000 women per year, and this incidence increases with age. The average age at diagnosis is in the 7th to 8th decades of life, with a future rise in absolute numbers of vulvar SCC expected, mainly due to the proportional increase in the average age of the general population .
Two different types of vulvar SCC have been delineated, each with their own precursors. The first type is associated with an infection with one of the high-risk types of Human Papillomaviruses (HPV), and it primarily affects younger women. The other type is associated with a lichen sclerosus (LS) condition, and it occurs predominantly in elderly patients independent of any HPV infection . Although the pathogenesis of vulvar SCC has been investigated, it has not been documented nearly as well as the pathogenesis of the more common cervical SCC.
We herein give an overview, focusing on the molecular events of these two distinct HPV-associated and independent pathways for the development of vulvar SCC.
2. Clinical and Pathological Features of SCC and Its Precursors
Vulvar SCC accounts for 90% of vulvar cancers and 5% of gynecological cancers. Patients usually present with a vulvar mass, which may be pruritic or painful or be associated with bleeding, and, occasionally, with a groin mass. Clinical factors that have adverse prognostic significance include increased stage, older age, smoking, and fixed or ulcerated groin nodes .
The three main histological subtypes of vulvar SCC are: warty, basaloid, and keratinizing (Table 1 and Figure 1). The predominant type, keratinizing, accounts for 65%–80% of vulvar SCCs; the basaloid and warty types of SCC account for the remaining 20%–35% . The keratinizing type usually occurs in postmenopausal women; the warty/basaloid types tend to occur more often in premenopausal or perimenopausal women. The keratinizing type is usually formed by well, or moderately differentiated cells with an absence of koilocytosis. There are usually one or more adjacent epithelial lesions, including LS, squamous cell hyperplasia (SCH), and differentiated vulvar intraepithelial neoplasia (VIN) , which will each be described further in the following sections.
(b) SCC, differentiated type
(c) SCC, warty type
(d) SCC, basaloid type
The warty or basaloid types of SCC often accompany a normal-type VIN. The basaloid type typically grows in bands, sheets, or nests within a desmoplastic stroma, and focal cytoplasmic maturation and keratinization may be observed. The warty type exhibits invasion as bulbous or irregular jagged nests, often with prominent keratinization. The koilocytotic tumor cells have pleomorphic to bizarre, often multiple, nuclei with irregular contours that vary from hyperchromatic and shrunken to those with clumped or smudged chromatin .
Other histological subtypes include verrucous carcinoma, giant cell carcinoma, and acantholytic squamous cell carcinoma. Verrucous carcinoma is highly differentiated squamous carcinoma that has a verrucous pattern and invades with a pushing border in the form of bulbous pegs of neoplastic cells. Squamous cell carcinoma with tumor giant cells is a variant of SCC characterized by multinucleated tumor giant cells, large nuclei with prominent nucleoli, and prominent eosinophilic cytoplasm. This variant is relatively rare and is associated with a poor prognosis. The acantholytic squamous cell carcinoma forms rounded spaces, or pseudoacini, lined with a single layer of squamous cells. Dyskeratotic and acantholytic cells are sometimes present in the central lumen .
Various terms have been used to define the precursors of vulvar SCC. Bowen first reported on these squamous intraepithelial lesions in 1912, and they are now commonly referred to as Bowen’s disease; since then, a myriad of clinical and histopathological terms have been employed to describe these vulvar precancerous lesions . The International Society for the Study of Vulvar Disease (ISSVD) simplified the terminology for carcinoma in situ and vulvar atypia in 1976; in 1986 they adopted the single term of VIN and a 3-grade VIN system based on the terminology from cervical intraepithelial neoplasia (CIN) . In VIN 1, maturation was present in the upper two-thirds of the epithelium. In VIN 2, the dysplasia involves the lower two-thirds of the epithelium, and in VIN 3, the dysplasia extends into the upper third . The terms of warty, basaloid, and differentiated (simplex) are used in the same way as for cervical SCC.
The most recent classifications are shown in Table 2. The World Health Organization (WHO) classifies VIN according to the 3-grade system for both the warty/basaloid types and the simplex type . In 2004, ISSVD modified their VIN terminology and suggested a 2-tier classification: VIN usual type and VIN differentiated type. Moreover, they decided to abolish the term VIN 1. The term of VIN is now applied only to the histologically high-grade squamous lesions that were the former VIN 2 and VIN 3 or differentiated VIN . This revision was made based on the observation that there was neither evidence that the morphologic spectrums of VIN 1, 2, and 3 reflect a biologic continuum nor that VIN 1 was a cancer precursor . In 2005, Medeiros et al. proposed a Bethesda-like grading system of low-grade vulvar intraepithelial lesions (Low-grade VILs) and high-grade vulvar intraepithelial lesions (High-grade VILs) . Low-grade VILs correspond to lesions associated with low-risk HPV infections. Condyloma acuminatum and discrete raised lesions with minimal atypia and lacking the features of dermatosis (VIN 1) were categorized into low-grade VIL .
|VIN1: abolished terminology.|
A systematic review of the progression rate from VIN 3 to invasive SCC, after various clinical treatments, was reported to be 3.3% . Nine percent of 88 untreated patients progressed to SCC during 12 to 96 months. Complete regression of usual VIN 3 lesions were observed in 1.2% of 3322 patients, mostly during the first 10 months after diagnosis, 41% of which remission was related to pregnancy. Another study demonstrated that the overall percentage of differentiated VIN lesions with subsequent diagnosis of SCC was 32.8%, and that of usual type VIN was 5.7% . The median time for progression from usual type VIN to SCC was 41.4 months whereas that from differentiated VIN to SCC was significantly shorter: 22.8 months (). Another study demonstrated that the mean time from the incidence of HPV infection to the development of VIN 1–3 was 18.5 months (95% confidence interval, 13.4–23.6) .
The typical presentation of usual VIN is a pruritic, burning, or asymptomatic, white, red, or pigmented lesion. The incidence of this form of VIN has almost doubled over the past several decades, with a significant increase in younger women. The lesions of differentiated VIN usually range from 0.5 to 3.5 cm, appearing as single or multiple gray-white areas with a rough surface or ill-defined white plaques or nodules. The lesions usually occur in postmenopausal women .
LS runs a relapsing and remitting course and presenting symptoms include pruritus, soreness, burning, and irritation. Typically, the lesions are white plaques and papules, often with areas of erythema hyperkeratosis, pallor, and ulcer .
The histological features of lichen sclerosus (LS) include a thinned epidermis with loss of normal rete pegs, basal layer vacuolar changes and a paucity of melanocytes, and, moreover, a wide band of homogeneous collagen below the dermatoepidermal junction and a band-like lymphocytic infiltrate below the homogenized area are present (Figure 1). The dermis often shows variable degrees of edema .
LS most commonly affects the anogenital area (85% to 98%), with extragenital lesions occurring in 15% to 20% of the patients . LS occurs at all ages; however, it has a bimodal peak of incidence in prepubertal girls and postmenopausal women . According to a previous study, 1 out of 30 elderly women suffer from LS . As association of LS with autoimmune disorders has been demonstrated. According to previous studies, around 30% of the patients have active autoimmune disease and autoantibodies were detected in about half of the serum of the LS patients [2, 13–16]. LS is considered to occur at sites of injured skin in women with the susceptible immunophenotype who scratch the area because of genital irritation .
The risk of development of vulvar SCC in women with LS was shown to be 4% to 5% . A previous review study also estimated a 4.5% frequency of SCC arising in LS with an interval of approximately 10 years (1.67 to 12.5 years) after diagnosis of LS without SCC .
3. Mechanisms of Carcinogenesis
3.1. HPV-Related Carcinogenesis
Two distinct pathways, HPV related and HPV independent, were proposed for vulvar carcinogenesis (Figure 2). Warty/basaloid type SCC develops through usual (warty/basaloid) type VIN triggered by infection with high-risk type HPVs, predominantly HPV16 and 18 . Usual type VIN lesions are observed adjacent to greater than 10%–67% of the vulvar SCC lesions . A previous study showed that 69% to 100% of warty/basaloid type SCC were positive for high-risk type HPVs . High-risk type HPVs were detected in 84% of 45 usual VIN cases . Eighty-seven percent (13 of 15) of the usual high-risk type HPV-positive VIN lesions were shown to express both p14 (a cell-cycle regulator which mediates p53 activation) and p16 (a cyclin-dependent kinase inhibitor) . Hoevenaars showed that all 38 usual VIN lesions exhibited positive immunohistochemical staining, and that in all these cases a high MIB1 index was observed. No expression of p53 and p16 was detected in normal epithelium of the vulva . However, so far little has been found concerning the mechanism of enhanced expression of p14 and p16 in the carcinogenesis of vulvar SCC.
The HPV viral gene products E6 and E7 interact with host cell p53 and Rb proteins, resulting in p53 dysfunction and inactivation of Rb, respectively. In cervical carcinogenesis triggered by high-risk type HPV infection, E7 inhibits Rb, resulting in the release of active host E2F-1, which positively regulates host p14. E6 inhibits p53 function by binding with E6-AP ubiquitin ligase, and leads to p14 upregulation via p53 degradation by negative feedback mechanism [22, 23]. Functional inactivation of Rb by E7 protein also leads to p16 overexpression. Taken together, p14 and p16 were over-expressed as a consequence of HPV E6 and E7 expression in cervical carcinomas.
In carcinogenesis of HPV-related SCC, similar mechanisms to cervical carcinogenesis seem to play an important role. Degradation and inactivation of the tumor suppressor genes p53 and Rb leads to absence of cell-cycle arrest and hyperproliferation of tumor cells. Frequent detection of over-expression of p14 and p16 in VIN suggests that degradation and inactivation of p53 and Rb are early events in carcinogenesis of HPV-related SCC of the vulva.
3.1.1. Integration of High-Risk Type HPV DNA
In uterine cervical carcinogenesis, integration of the high-risk type HPV DNA into the host genome was demonstrated to be an initial step for monoclonal expansion of dysplastic cells . In the process of the integration, some parts of the E2 open reading frame (ORF), which encode a 48-kd phosphorylated protein involved in the regulation of viral DNA transcription and replication, are usually disrupted or deleted from HPV genome, causing up-regulation of the oncogenic E6 and E7 genes . HPV integration sites were demonstrated to be semirandomly distributed over the whole genome, with a clear predilection for genomic fragile sites, but there was no evidence for targeted disruption or functional alteration of critical cellular genes by the integrated viral sequences. The main function of HPV integration is considered to be for the stabilization of viral oncogene transcription [26, 27].
Similar mechanisms to those for cervical cancer seem to play an important role during the development of HPV-related vulvar SCC triggered by high-risk type HPV infection. Integration of HPV-16 DNA, with deletion of the E2 ORF in both the SCC portion and its adjacent VIN 3 lesions, which were all implied to be formed from a single cell of origin through monoclonal expansion, was first demonstrated in a case of vulvar SCC by Ueda et al. . Monoclonal composition was also demonstrated in 3 of 7 cases of VIN 1/2, and 12 of 13 VIN 3 cases in the study. Later, additional supporting studies have also shown VIN 3 cases associated with infection of HPV in an integrated form [28, 29].
3.1.2. HPV Vaccine
The HPV vaccine is a tremendously promising new tool for the prevention of HPV-related SCC of the vulva, as it has already been for the cervix. The FUTURE I study has demonstrated that a prophylactic quadrivalent HPV-(6/11/16/18) L1 VLP vaccine significantly reduced the incidence of HPV-associated anogenital diseases in young women . The prophylactic efficacy in the study was 100% for vulvar condyloma, VIN 1, and VIN 2/3 in the per-protocol population. Other studies also demonstrated that the prophylactic quadrivalent HPV vaccine completely protected VIN 2/3 [31, 32].
Interestingly, a series of 3-4 vaccinations against a synthetic long-peptide of the HPV-16 oncoproteins E6 and E7 was shown to be therapeutically effective for HPV-16-positive VIN 3 patients . At 3 months after the last vaccination, 5 (25%) of 20 patients had complete remission of the lesion, and HPV-16 was no longer detected in 4 cases (20%). At 12 month of followup, 9 (47%) of 19 patients had a complete response with tolerable adverse effects. The patients who had a complete response at 3 months demonstrated a significantly stronger interferon--associated proliferative CD4 T-cell response and a broader response of CD8 interferon- T-cells. A phase II clinical trial of the topical immune-modulator imiquimod, followed by therapeutic HPV-16 vaccine using a fusion protein of HPV-16 E6E7L2 on 19 cases with VIN 2/3, demonstrated that complete regression of VIN 2/3 lesions was observed in 63% of the cases (12 of 19) .
3.2. HPV-Independent Carcinogenesis
The majority of vulvar SCC are considered to occur in elderly women through differentiated VIN in a background of LS . High-risk type HPVs were detected in none of 75 differentiated VIN cases . They also showed that all 75 differentiated VIN lesions exhibited negative p16 immunohistochemical staining, and a low MIB1 index was observed in 96% (72 of 75 cases) of the cases . No relationship between HPV infection and LS was found in these women [2, 21]. These results strongly suggest that an HPV-independent pathway exists for carcinogenesis of vulvar SCC from LS through differentiated VIN; however, the mechanism of HPV-independent carcinogenesis has not yet been fully elucidated.
We have previously demonstrated that 2 of 6 LS lesions exhibited monoclonality, implying that certain important molecular alterations might occur in some LS lesions well before histologically apparent malignant transformation to differentiated VIN or keratinizing SCC occurs . Rolfe et al. showed that 10 of 12 LS-associated SCCs exhibited a p53 mutation, and in 7 of those 10 cases LS lesions exhibited the p53 mutation at the same codon as in the SCC lesions, suggesting that a p53 mutation is possibly involved early in the HPV-independent pathway of vulvar carcinogenesis . Somatic mutation of PTEN was also demonstrated in some cases of vulvar SCC and VIN, suggesting that PTEN mutation possibly played a role early in the carcinogenesis of vulvar SCC . Pinto et al. found that an allelic imbalance (AI) was present in 67%, 53%, and 43% of usual type VIN, differentiated VIN and LSs, respectively, and that microsatellite instability (MSI) was detected in 3 (20%) of 15 differentiated VIN, and 2 (12%) of 17 LS, but none of usual type VIN, implying that these molecular alterations are also possibly early events in vulvar carcinogenesis, and that MSI may play a critical role for malignant potential of LS .
A recent study demonstrated more frequent hypermethylation of RASSF2A, MGMT, and TSP-1 genes in SCC associated with LS than in SCC not associated with LS, suggesting a possible role of these genes in HPV-independent carcinogenesis .
A fraction of squamous cell hyperplasia (SCH) lesions were shown to be monoclonal in composition  and p53 mutation, AI, and MSI were observed in 22%, 50% and 20%, respectively, of SCH cases [36, 38]. SCH with atypia might be a precursor of SCC; however, SCH without atypia is, currently, not regarded as a direct precursor of SCC. Relationship between SCH and keratinizing SCC is still to be determined [2, 38].
Two distinct pathways leading to vulvar SCC have been suggested. One is a pathway primarily linked to infection with high-risk types of HPV; the other is an HPV-independent scenario. Mechanisms similar to those that drive cervical carcinogenesis possibly play an important role in HPV-related carcinogenesis of vulvar SCC. HPV prophylactic and therapeutic vaccines are both promising to prevent HPV infection and prevent development of warty/basaloid type SCC from its precursor, the usual type VIN. On the other hand, keratinizing type vulvar SCC, which by far represents the majority of vulvar SCC, occurs independently from HPV infection in a background of LS. The mechanism of carcinogenic progression forward from LS in this second pathway has not fully delineated, and it is not yet clear whether medical treatments of LS prevent malignant transformation. Further clinical and basic research into these important areas is still required.
|CIN:||Cervical intraepithelial neoplasia|
|ISSVD:||The International Society for the Study of Vulvar Disease|
|SCC:||Squamous cell carcinoma|
|SCH:||Squamous cell hyperplasia|
|VIL:||Vulvar intraepithelial lesions|
|VIN:||Vulvar intraepithelial neoplasia|
|WHO:||World Health Organisation.|
The authors would like to thank Gregory S. Buzard, CDCP, for his constructive critique of our paper.
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