Figure 2: Curcumin’s effects on AKT/MTOR and ERK pathways in vitro. (a) Western blot of SRB12-p9 tumor cells treated with (+) or without (−) 20 μM curcumin for 24 hours and probed with the indicated antibody. Representative Western blots for two analyzed sets are shown. (b) Band densities of indicated biomarkers ( ) were quantified using ImageQuant software and normalized to actin protein level. Data presented as Mean ± SE. * Indicates versus vehicle-treated control. A significant inhibition of expression of the following biomarkers was observed: pAKT ( ); pS6 ( ); p4EBP1 ( ); pSTAT3 ( ); pERK1/2 ( ). a.u.: arbitrary units.