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147863.fig.003b
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Figure 3: Curcumin’s effects on the ERK pathway in vivo. (a) Western blot of pooled xenograft tumors ( ) of the indicated antibody. (b) The presence and intensity of pERK staining (brown) in the control group compared to the presence and intensity of pERK staining in the curcumin-treated xenograft tumors. (c) Representative IHC staining of SRB-12 p9 WT cell tumor xenografts. Paraffin sections of tumors were probed with STAT3 phospho-Tyr705 (pSTAT3, top row) or ERK1/2 phospho-Thr202/Tyr204 (pERK, bottom row), followed by an Alexa546-labeled secondary antibody (400x). (d) IL-6 ELISA of pooled mouse serum ( ) in duplicate.