Genome-Wide Analysis of Gene and Protein Expression of Dysplastic Naevus Cells

Table 3

Top 16 differentially expressed proteins between melanocytes from dysplastic naevus and from normal skin.

Swiss-prot id	Protein name	Mol. mass calculated (Da)^1	𝑝𝐼 obs.^2	𝑝𝐼 calc.^3	% Pept. Coverage^4	Ratio; (DNMC/MC)^5	𝑃 value	General function
Q9Y2R9	Ribosomal protein S7	22113	10.09	10	43	0.1310	0.010946	Translational elongation
P32969	Ribosomal protein L9	21964	9.96	9.96	29	0.22334	0.016167	Translational elongation
P78417	Glutathione S-transferase omega 1-1	27833	6.23	6.24	21	0.23627	0.013815	Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities
P62753	Ribosomal protein S6	28842	10.9	10.85	23	0.31890	0.012139	Controlling cell growth and proliferation through the selective translation of particular classes of mRNA
O75947	ATP synthase, H^+ transporting, mitochondrial F0 complex, subunit d	18537	5.21	5.21	41	0.32135	0.027965	Mitochondrial membrane ATP synthase that produces ATP from ADP
P04792	Heat shock protein 27	22427	7.83	5.98	57	0.36622	0.023047	Involved in stress resistance and actin organization
P02794	Ferritin heavy chain	21252	5.3	5.08	46	0.40067	0.006873	Stores iron in a soluble, nontoxic, readily available form. Important for iron homeostasis
Q59EQ2	Tyrosine 3-monooxy-genase/tryptophan 5-monooxygenase activation protein	28179	4.76	4.73	26	0.41836	0.012436	Oxidoreductase
P08670	Vimentin	53710	5.06	5.06	43	0.4478	0.023374	Vimentins are class-III intermediate filaments
P07355	Annexin A2	36631	8.32	7.56	53	0.47142	0.00642	May be involved in heat-stress response
P47985	Cytochrome b-c1 complex subunit Rieske	29934	8.55	6.3	5	0.47725	0.008578	Component of the ubiquinol-cytochrome c reductase complex
Q5U0F4	Eukaryotic translation initiation factor 3	36878	5.38	5.38	32	3.2155	0.018194	Protein synthesis
O94844	GTP-binding Rho-like protein cdc42	20123	5.25	4.83	55	2.88699	0.017896	The protein may play a role in small GTPase-mediated signal transduction and the organization of the actin filament system
P07237	Prolyl 4-hydroxylase	57480	4.76	4.69	46	2.79651	0.009987	This multifunctional protein catalyzes the formation, breakage, and rearrangement of disulfide bonds
Q6IBR6	Platelet-activating factor acetylhydrolase	25569	5.57	5.57		2.46963	0.008975	Lipid metabolic process
Q9UBS4	DnaJ (Hsp40) homolog, subfamily B	40774	5.81	5.81	30	2.33737	0.011076	Binds directly to both unfolded proteins that are substrates for ERAD and nascent unfolded peptide chains

¹Calculated molecular mass of the protein in Dalton (Da).
²Isoelectric point (𝑝_𝐼) observed in this study.
³𝑝_𝐼 calculated according to the Mascot database.
⁴Percentage peptide coverage.
⁵Protein ratio melanocytes from dysplastic naevus (DNMC)/melanocytes from normal skin (MC).