Potential Role of Meiosis Proteins in Melanoma Chromosomal Instability

Figure 2

Analysis of meiosis proteins by immunofluorescence. Immunofluorescence was used to analyze the expression of SPO11, REC8, SGO2, and HORMAD1 in the DM2N melanoma line and nontransformed melanocytes using the same antibodies listed in Figure 1. Anti-rabbit secondary antibody was used for SPO11, REC8, and SGO2 and anti-mouse secondary was used for HORMAD1. The staining patterns varied between the different antibodies. SPO11, REC8, and SGO2 stained in a predominately nuclear manner with SPO11 demonstrating a fibular, dotted pattern, REC8 with discrete nuclear dots, and SGO2 with diffuse staining throughout the nucleus. HORMAD1 had a mostly cytoplasmic staining pattern although nuclear staining was also visualized. With all four antibodies, the staining in the melanoma was markedly stronger than the melanocytes.