Melanoma-Targeted Chemothermotherapy and In Situ Peptide Immunotherapy through HSP Production by Using Melanogenesis Substrate, NPrCAP, and Magnetite Nanoparticles

Figure 3

Depigmenting effect of NPrCAP. (a) Depigmentation of C57 black mouse hair follicles by a single ip administration of NPrCAP or NAcCAP results in complete loss of melanin pigmentation. Entire coat color changes to silver from black. Electron microscopic observation reveals selective degradation of melanocytes and melanogenic organelles such as early-stage melanosomes at 6 hr after administration. At 24 hr after administration, these melanocytes reveal total degradation. (b) Depigmentation of black skin after topical application of NPrCAP. There is a marked decrease of melanocyte populations after topical application. Electron microscopic observation indicates selective accumulation of NPrCAP in the tyrosinase areas such as in melanosomes and Golgi apparatus as indicated by the deposition of electron dense materials (see arrows).

(a) Depigmentation of C57 black mouse hair by a single ip administration of NPrCAP or NAcCAP  

(b) Depigmentation of black skin by topical NPrCAP