Research Article

Kaposi’s Sarcoma-Associated Herpesvirus Subversion of the Anti-Inflammatory Response in Human Skin Cells Reveals Correlates of Latency and Disease Pathogenesis

Figure 4

Differential expression of LANA in KSHV-infected MeWo and Mel1700 cells reveals diffuse nuclear staining as a marker of spontaneous or drug-induced lytic replication. Infected MeWo-KSHV (a) and Mel1700-KSHV cells (b) were plated in chamber slides and allowed to adhere overnight, then fixed, permeabilized, and stained with the LANA-specific LN35 primary antibody, followed by chicken anti-rat secondary antibody conjugated to Alexa-Fluor-647 (Cy5). Shown are 63x magnification (oil immersion) images of infected MeWo-KSHV (a) and Mel1700-KSHV cells (b) showing LANA (top image) along with the corresponding DAPI nuclear counter-stain (bottom image), respectively. Note the predominantly discrete “punctate” LANA staining in MeWo-KSHV cells (a) in contrast to the more diffuse LANA staining in almost all Mel1700-KSHV cells (b).
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