Kaposi’s Sarcoma-Associated Herpesvirus Subversion of the Anti-Inflammatory Response in Human Skin Cells Reveals Correlates of Latency and Disease Pathogenesis
Figure 5
Differential expression of specific LANA isoforms in KSHV-infected MeWo and Mel1700 cells is associated with differences in viral lytic replication. (a) RIPA buffer lysates of rKSHV.219-infected MeWo and Mel1700 cells either untreated (−) or treated with NaB (+) for four days were resolved in SDS-PAGE and probed with anti-LANA antibody LN35. Blots were visualized with anti-rat-horse radish peroxidase (HRP)-conjugated secondary antibody. (b) 30 sec, 5 min., and 30 min. exposure times for the blot in (a), revealing “fine” differences between the samples with respect to expression of specific LANA bands. β-Actin (30 sec. exposure) was used as loading control, and the estimated LANA band sizes are on the right side of the blot(s).
