Representative example of histology of Merkel cell carcinoma from a tumor with poor prognosis. (a) Low power hematoxylin and eosin (H&E) stained photomicrograph. Arrows indicate regions of MCC to be microdissected. Dermis (De), epidermis (Ep), and sebaceous gland (SGI) are also indicated. (b) High power H&E image of same MCC. (c) Arcturus glass membrane hematoxylin counterstained serial section from same MCC. Areas to be laser capture microdissected are indicated by the arrow and red shading. The red punctate shading marks subsequent laser positioning. Using the Microdissection System (molecular devices), these regions were transferred onto CapSure HS LCM Caps (Molecular Devices). Four caps were used per patient sample from the dissection of 1–4 sections representing approximately 5,000 tumor cells (1-2 mm2 area) captured per cap. (d) Image of the same membrane cut section after LCM. Magnification: (a) at 2X; (b), (c), and (d) at 10X. Scale bar = 50 μm.
