Journal of Skin Cancer / 2014 / Article / Fig 2

Research Article

Melanocyte and Melanoma Cell Activation by Calprotectin

Figure 2

Melanocyte and melanoma cell proliferation and migration in response to calprotectin. (a) NHM or WC62 cells were plated in 96-well plates, treated with a mixture of recombinant S100A8 (100 pg/mL) and S100A9 (1 ng/mL), and allowed to dimerize in vitro. Cell number was determined using the MTT assay. Triplicate samples were run for each treatment in each experiment and the experiments were repeated 5 times. Bars indicate SEM. Asterisks indicate a significant difference () between control and calprotectin-treated cells as determined by 2-tailed Student -test assuming unequal variance. (b) NHM or WC62 cells were plated in uncoated or Matrigel-coated transwell chambers containing the appropriate growth medium. Lower chambers were filled with medium alone or with medium containing recombinant S100A8 (100 pg/mL) and S100A9 (1 ng/mL) (Abnova, Taiwan) and were allowed to dimerize in vitro. Twenty-four hours later, the number of cells that had migrated through the membrane was determined as described in the text. Three replicate experiments were performed. Bars indicate SD. Asterisks indicate a significant difference () between control and calprotectin-treated cells as determined by 2-tailed Student -test assuming unequal variance.
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