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Volume 13 (1997), Issue 2, Pages 131-136

Combination of capillary GC, GC/MS and 13C-NMR for the characterization of the rhizome oil of Piper betle L. (Piperaceae) from Vietnam

Lê Thanh,1 Nguyên Xuân Dung,2 Ange Bighelliand,3 Joseph Casanova,3 and Piet A. Leclercq4

1Huê University, 34 Le Loi, Huê, Vietnam
2Center for Education and Development of Chromatography, EDC Vietnam, 3 Giai Phóng Street, Hai Bà District, 10000 Hanoi, Vietnam
3Université de Corse, Equipe Chimie et Biomasse, CRES-URA CNRS 2053, Route des Sanguinaires, 20000 Ajaccio, France
4Laboratory of Instrumental Analysis, Department of Chemical Engineering, Eindhoven University of Technology, P.O. Box. 513, 5600 MB Eindhoven, The Netherlands

Received 1 June 1996; Revised 29 August 1996

Copyright © 1997 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The essential oil from the rhizomes of Piper betle L. (betel), collected around Hue, was obtained in 0.20% yield. The oil was examined by a combination of capillary GC and GC/MS. 13C-NMR studies confirmed the structure assignments proposed by retention data and mass spectra of the components with a content higher than 1%. In some instances the structure elucidation based on GC and GC/MS data had to be corrected. More than forty constituents were found of which the major ones were α-cadinol (26.2%), δ-cadinene (11.7%), and about equal amounts of T-cadinol and T-muurolol (unseparated, together 20.7%). This study clearly illustrates the advantage of complementary identification techniques.