Journal of Spectroscopy

Journal of Spectroscopy / 2003 / Article

Open Access

Volume 17 |Article ID 123586 | https://doi.org/10.1155/2003/123586

Eriko Mano, Hironari Kamikubo, Yasushi Imamoto, Mikio Kataoka, "Comparison of the Photochemical Reaction of Photoactive Yellow Protein in Crystal with Reaction in Solution 1", Journal of Spectroscopy, vol. 17, Article ID 123586, 9 pages, 2003. https://doi.org/10.1155/2003/123586

Comparison of the Photochemical Reaction of Photoactive Yellow Protein in Crystal with Reaction in Solution 1

Abstract

Photoactive yellow protein (PYP) is a photoreceptor protein for the negative phototaxis of Ectothiorhodospira halophila. The crystal structures of several photo‒intermediates have been revealed by X-ray crystallography. In the crystal structure of the active intermediate, PYPM, no significant structural changes were observed except for the vicinity of the chromophore. On the contrary, spectroscopic studies with solution condition demonstrated that global structural changes occur during the photo‒cycle. In order to reveal the origin of the discrepancies, we measured the reaction kinetics upon illumination under crystal condition and to compare them with those observed under solution condition. The reactive portion decreases with the increase of crystallinity. The rate constant of PYPM decay also decreases with the increase of crystallinity. These results suggest two possibilities: (1) PYP in crystal does not react by the illumination; (2) the photoreaction rate is highly accelerated in crystal. Consequently, the photoreaction in crystal is considered to be highly influenced by the force constraint from crystalline lattice.

Copyright © 2003 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


More related articles

 PDF Download Citation Citation
 Order printed copiesOrder
Views114
Downloads321
Citations

We are committed to sharing findings related to COVID-19 as quickly as possible. We will be providing unlimited waivers of publication charges for accepted research articles as well as case reports and case series related to COVID-19. Review articles are excluded from this waiver policy. Sign up here as a reviewer to help fast-track new submissions.