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Volume 18 (2004), Issue 2, Pages 251-256

Probing the role of metal ions on reversible peptide–protein interactions by NMR

Nicola D'Amelio, Elena Gaggelli, Nicola Gaggelli, Francesca Mancini, Elena Molteni, Daniela Valensin, and Gianni Valensin

Department of Chemistry and the NMR Center, University of Siena, Via A.Moro, Siena 53100, Italy

Copyright © 2004 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This work provides evidence that paramagnetic lanthanide ions constitute ideal probes suitable for investigations of metal effects upon peptide–receptor interactions with the use of NMR methods. Cerium(III) is herein used for assessing metal effects upon the interaction between angiotensin II and a fragment from the AT1A receptor. Angiotensin II forms a complex with cerium(III) in water while the fCT300–320 receptor fragment is poorly affected by cerium(III). However, the addition of the fragment displaces cerium(III) from the complex, thus directly demonstrating the higher affinity of angiotensin II for the receptor and probing the peptide residues involved in receptor binding.