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Spectroscopy
Volume 19 (2005), 5,6, Pages 259-266
http://dx.doi.org/10.1155/2005/258617

Affinity studies of hypocrellin B and mono-cysteine substituted hypocrellin B with CT-DNA using spectroscopic methods

Shaohua Wei,1,2 Jiahong Zhou,2 Yuying Feng,2 Deyin Huang,1 Xuesong Wang,2 Baowen Zhang,3 and Jian Shen2

1School of Chemistry and Chemical Technology, Shanghai JiaoTong University, Shanghai 200240, China
2Jiangsu Engineering Research Center for Bio-medical Function Material, Nanjing Normal University, Nanjing 210097, China
3Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100101, China

Copyright © 2005 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The interaction of anticancer drug hypocrellin B (HB) and mono-cysteine substituted hypocrellin B (MCHB) with calf thymus deoxyribonucleic acid (CT-DNA) has been investigated using spectral methods. The results of UV–visible spectra showed that the HB and MCHB can intercalate into the base-stacking domain of the CT-DNA double helix. Further studies based on fluorescence spectroscopy and circular dichroism (CD) spectroscopy also supported the intercalation mechanism.