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Spectroscopy
Volume 20, Issue 2, Pages 45-55
http://dx.doi.org/10.1155/2006/359876

Application of a low-angle light scattering technique to cell volume and cell signaling studies on Ehrlich ascite tumor cells

Valeriy P. Zinchenko,1 Vyacheslav V. Lee,1 Alexey V. Berezhnov,1 Igor V. Mindukshev,2 Richard O. Jenkins,3,5 and Nikolay V. Goncharov4

1Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino, Russia
2I.M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St-Petersburg, Russia
3School of Allied Health Sciences, De Montfort University, Leicester, UK
4Research Institute of Hygiene, Occupational Pathology and Human Ecology, St-Petersburg, Russia
5School of Allied Health Sciences, De Montfort University, Leicester, LE1 9BH, UK

Copyright © 2006 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A method for studying cells based on low-angle light scattering was applied to cell volume and cell signaling studies on Ehrlich ascite tumor cells (EATC). Changes in the volume of EATC were measured in hypotonic medium, as well as after activation with exogenous ATP, ionomycin and thimerosal. Increase of [Ca2+]i under ATP and ionomycin action induced reversible changes of cell volume: fast shrinking was followed by swelling. Thimerosal caused a reversible change in EATC volume with high amplitude; endoplasmic reticulum played the key role in this response. Having obtained kinetic parameters of changes in cell volume under activation of the cells, quantitative measurements of K+, Na+ and anion flows responsible for this process can then be obtained. In spite of some fundamental differences in the behavior of cells of different dimensions there are many similarities, and there is a good theoretical background for dealing with both small and large cells.