Journal of Spectroscopy

Journal of Spectroscopy / 2008 / Article

Open Access

Volume 22 |Article ID 715905 | https://doi.org/10.3233/SPE-2008-0361

Grace O'Maille, Eden P. Go, Linh Hoang, Elizabeth J. Want, Colin Smith, Paul O'Maille, Anders NordstrÖm, Hirotoshi Morita, Chuan Qin, Wilasinee Uritboonthai, Junefredo Apon, Richard Moore, James Garrett, Gary Siuzdak, "Metabolomics relative quantitation with mass spectrometry using chemical derivatization and isotope labeling", Journal of Spectroscopy, vol. 22, Article ID 715905, 17 pages, 2008. https://doi.org/10.3233/SPE-2008-0361

Metabolomics relative quantitation with mass spectrometry using chemical derivatization and isotope labeling

Abstract

Comprehensive detection and quantitation of metabolites from a biological source constitute the major challenges of current metabolomics research. Two chemical derivatization methodologies, butylation and amination, were applied to human serum for ionization enhancement of a broad spectrum of metabolite classes, including steroids and amino acids. LC-ESI-MS analysis of the derivatized serum samples provided a significant signal elevation across the total ion chromatogram to over a 100-fold increase in ionization efficiency. It was also demonstrated that derivatization combined with isotopically labeled reagents facilitated the relative quantitation of derivatized metabolites from individual as well as pooled samples.

Copyright © 2008 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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