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Spectroscopy
Volume 23 (2009), Issue 5-6, Pages 257-263
http://dx.doi.org/10.3233/SPE-2009-0396

The application of two-dimensional fluorescence correlation spectroscopy on the interaction between bovine serum albumin and prulifloxacin

Mao-Yun Xue,1,2 Ai-Ping Yang,1,2 Mei-Hua Ma,1,2 and Xiao-Hua Li1,2,3

1Department of Engineering Technology, Jiangsu Institute of Economic and Trade Technology, Nanjing, China
2Research and Development Centre of Food Safety Engineering Technique of Jiangsu, Nanjing, China
3Research and Development Centre of Food Safety Engineering Technique of Jiangsu, Nanjing 210007, Jiangsu Province, China

Copyright © 2009 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The interaction between bovine serum albumin (BSA) and prulifloxacin was investigated by ultraviolet spectrophotometer (UV) and fluorescence spectroscopy in this paper. Two-dimensional (2D) correlation spectroscopy was applied to the analysis of fluorescence spectra. The results of spectroscopic measurements suggested that prulifloxacin (PL) have a strong ability to quench the intrinsic fluorescence of bovine serum albumin through static quenching procedure. Thermodynamic parameter enthalpy changes (ΔH) and entropy changes (ΔS) were calculated. Owing to the spectral resolution enhancement in 2D correlation spectroscopy, the structure change of prulifloxacin can be observed.