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Volume 23 (2009), Issue 5-6, Pages 291-297

Unique spectral features of DNA infrared bands of some microorganisms

M. Khalique Ahmed,1,2 Frank Amiama,1 and Earl A. Sealy1

1Department of Science, College of Arts and Sciences, Lynn University, Boca Raton, FL, USA
2Department of Science, College of Arts and Sciences, Lynn University, 3601North Military Trail, Boca Raton, FL-33431, USA

Copyright © 2009 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Half dozen microorganisms (Serratia marcescens, Klebsiella pneumonia, Staphylococcus aureus, Bacillus subtilis, Mycobacterium smegmatis and Saccharomyces cerevisiae) were cultivated, and their infrared spectra were recorded in the attenuated total reflection mode. Both the intensities and frequencies of the B-DNA and Z-DNA bands in the spectral region of 1250–900 cm–1 were found to be different for different microorganisms. We suggest that characterization and identification of microorganisms may be possible through analysis of their DNA bands only without the analysis of the full range (4000–700 cm–1) of their infrared spectra.