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Volume 23 (2009), Issue 3-4, Pages 209-216

Flow injection chemiluminescence determination of levofloxacin in medicine and biological fluids based on its enhancing effect on luminol–H2O2 reaction

Xiaodong Shao,1,4 Ying Li,2 Yangqin Liu,1 and Zhenghua Song3

1Tubular Goods Research Center of China National Petroleum Corporation, Xi'an, China
2Xi'an Thermal Power Research Institute Co. Ltd., Xi'an, China
3Northwest University, Xi'an, China
4Tubular Goods Research Center of China National Petroleum Corporation, Xi'an 710065, China

Copyright © 2009 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Levofloxacin{(–)-(S)-9-fluoro-2,3-dihydro-3-methyl-10-(4-methyl-1-piperazinyl)-7-oxo-7H-pyrido[1,2,3-de]-1,4-benzoxazine-6-carboxylic acid} is a synthetic fluorinated quinolone derivative, having activity against both Gram (+) and Gram (–) bacteria (aerobic and anaerobic) through inhibition of their DNA gyrase. In this paper, a simple flow injection chemiluminescence with luminol–hydrogen peroxide system was described for determining levofloxacin. The chemiluminescence intensity in the presence of levofloxacin was remarkably enhanced compared with that in the absence of it. Under the optimum reaction conditions the chemiluminescence increment produced was proportional to the concentration of levofloxacin in the range of 1.0–700.0 ng ml–1 (R2═0.9992), with a detection limit of 0.3 ng ml–1 (3σ). At the flow rate of 2.0 ml min–1, the whole process including sampling and washing could be completed in 0.5 min offering the sampling efficiency of 120 times h–1 accordingly, and the relative standard deviation (RSD) was less than 3.0% (n═5). The recovery for the levofloxacin samples was from 95.9% to 104.5%. It was satisfactory for the application to determine levofloxacin in pharmaceutical preparations, human urine and serum samples.