Journal of Spectroscopy

Journal of Spectroscopy / 2010 / Article

Open Access

Volume 24 |Article ID 576151 | https://doi.org/10.3233/SPE-2010-0474

Willem F. Wolkers, Harriëtte Oldenhof, "In situ FTIR studies on mammalian cells", Journal of Spectroscopy, vol. 24, Article ID 576151, 10 pages, 2010. https://doi.org/10.3233/SPE-2010-0474

In situ FTIR studies on mammalian cells

Abstract

In this work, we describe the application of FTIR spectroscopy to study lipids and proteins, in cellular systems during heating and cooling. Various spectral analysis methods are described to simultaneously study membrane phase behavior and heat-induced protein denaturation in cells. Membrane phase behavior was studied by following the temperature dependence of the CH2 stretching vibration bands. Protein denaturation was studied using the amide-I and -III bands. Both amide-I and amide-III band analysis show that heat-induced denaturation of proteins within the cells is typically associated with an increase in extended β-sheet structures and a concomitant decrease in α-helical structures.

Copyright © 2010 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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