From Molecule to Tissue: XIII European Conference on the Spectroscopy of Biological Molecules, Palermo, Italy, August 28–September 2, 2009, Part 2 of 2View this Special Issue
Petr Praus, Eva Kocišová, Peter Mojzeš, Josef Štepánek, Franck Sureau, Pierre-Yves Turpin, "Frequency domain fluorescence microspectrometry: Application to cellular uptake and drug distribution", Journal of Spectroscopy, vol. 24, Article ID 581520, 5 pages, 2010. https://doi.org/10.3233/SPE-2010-0436
Frequency domain fluorescence microspectrometry: Application to cellular uptake and drug distribution
Time-resolved confocal microspectrofluorometry and fluorescence microimaging were used to monitor how the model antisense oligonucleotide is transported into 3T3 living cells and distributed inside them. Phosphorothioate analog of 15-mer oligothymidylate labeled by ATTO 425 was complexed with Zn(II) 5,10,15,20-tetrakis(4-N-methylpyridyl) porphyrin as an uptake-mediating agent. Homodyne phase-resolved technique based on a high frequency analog modulation of both exciting diode laser and detector image intensifier was used for time-resolved measurements. Decay-time data obtained within a broad range spectral region have provided unique information about the fate of both fluorophores inside the cell.
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