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Spectroscopy
Volume 26, Issue 6, Pages 357-365
http://dx.doi.org/10.3233/SPE-2012-0564

Thermodynamic and spectroscopic study on the binding of interaction anionic phthalocyanine with calf thymus DNA

Hamid Dezhampanah,1,2 Termeh Darvishzad,1 and Mehrnaz Aghazadeh1

1Laboratory of Physical Chemistry, Department of Chemistry, Faculty of Science, University of Guilan, Rasht, Iran
2Laboratory of Physical Chemistry, Department of Chemistry, Faculty of Science, University of Guilan, P.O. Box 1914, Rasht-0098, Iran

Copyright © 2011 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The interaction between anionic form of copper (II) N,N',N",N'"-tetrasulfonated phthalocyanine Cu (tspc) and to calf thymus deoxyribonucleic acid (ct-DNA) is investigated by measuring UV-vis absorption and fluorescence spectroscopy in phosphate buffer. The binding constant and stoichiometry were determined by analysis of optical absorption spectra of phthalocyanine at various ct-DNA concentrations using SQUAD software. The static mode of fluorescence quenching of phthalocyanine by calf thymus deoxyribonucleic acid indicates the formation of a ground-state complex. The formation of ground-state complex is a spontaneous molecular interaction procedure in which outside groove binding through the formation of an axial bond between the base pairs of nucleotide and Cu in the central core of phthalocyanine.